Proteomic Profiling Of L6 Cell Lines to Identify Proteins That Can Serve As Potential Targets for Therapeutic Intervention of Type 2 Diabetes

Abstract

Type 2 Diabetes (T2D) is a metabolic disease characterized by abnormally high levels of sugar (glucose) in the blood (Hyperglycemia) which affects glucose homeostasis. It is caused due to the inability of the target cells to respond to insulin. Proteomics is the large-scale study of protein with their structures, functions, and information coded in a cell which is expressed and regulated at the protein level to achieve the function of an organism. Protein expression during the exposure of the cells to different glucose concentrations may alter and can give vital information about the pathogenesis of T2D. Study of underlying molecular mechanisms that lead to the development of T2D is important for the prevention and treatment of the disease the objective here is to study the effect of different glucose concentrations on proteomic profile of L6 cell lines. For this L6 skeletal muscle cells were exposed to different glucose (G) concentrations (0mM, and 25mM) for 48 hours. Total cell protein was extracted and protein profile was studied using SDS-PAGE. The identified proteins may provide information about disease pathophysiology and can serve as potential targets for therapeutic intervention of T2D.