Phytochemical screening, GCMS, UV-vis and FTIR analysis of leaf methanolic extract of Sapindus mukorossi L

Abstract

Sapindus mukarossi L is a medicinal plant comprising health-promoting secondary metabolites. The present study was aimed to analyses the phytochemicals through preliminary phytochemical screening and also to profile bioactive compounds in the methanolic extract of S. mukorossi leaves using GCMS, UV-vis, FTIR. Thin-layer chromatography (TLC) was carried out for the comparative metabolite profiling of the extracts using Chloroform: Methanol (90:10v/v) as a solvent system. The preliminary phytochemical screening tests revealed the presence of alkaloids, flavonoids, phenols, saponins, terpenoids, carbohydrates. The GCMS analysis of methanolic leaf extract was performed to identify the various bioactive compounds based on separation and concentration. FTIR analysis was done to identify the functional groups present in the plant extract. The UV-vis profile showed the presence of peaks which reveals the presence of secondary metabolites. From this study, it is concluded that the plant species contain effective phytochemical compounds, needs further research on toxicological aspects to develop a safe drug.