Genetic Diversity Analysis of Kabuli Chickpea (Cicer Arietinum L.) Promising Genotypes Using Molecular Marker

Abstract

The present investigation was carried out to study molecular diversity of different 20 kabuli Chick pea genotypes using PCR based molecular markers to find out the phylogenic relationship among them. In this study, 21 RAPD, 15 ISSR and 15 SSR primers were used to observe the relationship and polymorphism of kabuli Chick pea genotypes. Out of 21 RAPD primers 10 has generated 68 polymorphic bands with an average of 1.64 bands with 100% polymorphism per primer. The 15 ISSR primers engendered 82 bands in which 80 bands were polymorphic with 75 shared and 5 unique bands that were equal to 100% polymorphism with an average of 5.3 bands per primer. Total of 20 SSR primers were tested, with 12 of them amplifying a total of 14 bands out of them 7 polymorphic and 7 monomorphic bans having 22 shared and 1 unique band with 75.55% polymorphism with an average of 1.5 bands per primer. The similarity coefficient of clusters analysis ranged from 69 to 94% for RAPD, 65 to 97 % for ISSR and 66 to 75% for SSR. dendrogram which divided the genotypes into two main clusters I and II with an average resemblance of 77 %. Cluster I consist of all 19 Genotypes while cluster II consist only one genotype (ICCV-191308) which is most variable from all the genotypes. The pooled data of RAPD, ISSR and SSR generated clustering pattern appeared to be similar as RAPD clustering pattern. Based on the molecular markers associated with coconut genotypes, it was concluded that all molecular markers like RAPD, ISSR and SSR are most reliable to distinguish kabuli Chick pea genotypes.